克里蒙納名琴及中國古琴的特殊音色一直以來都是科學家想要了解的問題，過去的研究中曾經提到克里蒙納名琴上的塗漆或許是提琴音色的關鍵，但是在最近的研究中卻發現到塗漆似乎不是決定音色的主要因素，因此漸漸地認為製作名琴的木材所使用的化學處理或許才是特殊音色的關鍵。從中國古琴的研究中也發現古代的中國製琴師，常以特殊的方式對木材進行加速老化，以此來改變木材中的震動性質使製作出來的古琴音色更加優美。 本實驗的研究中，為了能夠了解克里蒙納名琴與化學處理過的雲杉樣本有何差異，以及年代更加久遠的中國古琴和現代木材有何差異，因此本實驗利用雙光子螢光超光譜顯微術獲得樣本的一維光譜資訊與二維的空間資訊，以此來分析在相同結構下各組樣本光譜基底的含量比例，從而了解各組樣本木質素的螢光有何變化;同時，藉由線性分離法將二倍頻訊號分離，以二倍頻訊號分析木材中纖維素的含量及分布情形。最後我們從各組樣本間的分析中發現克里蒙納名琴可能受到類似鹼性的化學影響，而中國古琴則是發現受到溫度影響的可能性非常高。 ;The unique timbre of the famous Cremona and Chinese guqin has always been a problem that scientists want to solve. In past research, it has been mentioned that the varnish on the famous Cremona may be the key to the unique timbre of the violin. But, recently In research, found that varnish does not seem to be the main factor in determining the tone, so it was gradually believed that the chemical treatment of the wood used to make the famous violin may be the key to the unique timbre. From the study of the Chinese guqin, it is also found that ancient Chinese luthiers often uniquely accelerated the aging of wood, to change the vibration properties in the wood to make the guqin sound more beautiful. In the research of this experiment, to understand the difference between the famous Cremona and the chemically treated spruce samples, as well as the difference between the ancient Chinese guqin and modern wood, this experiment uses two-photon fluorescence Spectral microscopy to obtain the one-dimensional spectral information and two-dimensional spatial information of the samples, to analyze the content ratio of the spectral bases of each group of samples under the same structure, so as to understand the changes in the fluorescence of lignin in each group of samples, The second harmonic generation is separated by the linear separation method, and second harmonic generation analyzes the content and distribution of cellulose in wood. Finally, from the analysis of each group of samples, we found that the Cremona may be affected by chemical effects similar to alkaline, while the Chinese Guqin is very likely to be affected by temperature.
