蛋白質體學在研究生物細胞與病理等相關機制以及診斷癌症的生物標記上扮演一重要角色。然而，蛋白質樣本稀少複雜，傳統分析方式費力耗時、高成本且低效率。使得蛋白質檢測為一艱鉅的任務。因此，開發一高效率、高靈敏度的生物分子檢測平台克服此技術瓶頸為本計畫之主要目標。本計畫提案提出一新型的生物分子檢測平台稱為「微流體分離-質譜分析晶片(S-MS chip)」，結合樣本分離、質譜結合與無基質質譜分析技術於單一微型晶片上，達到簡易、高靈敏與高通量之分析方式。計畫分為三大研究方向執行：（I）微流體分離-質譜分析晶片製程開發與製作。此部份目標以金屬輔助化學蝕刻法製備高表面積/面積比與通透性之矽奈米結構用於晶片分離與質譜分析。（II）微流體分離-質譜分析晶片之表面處理以及微流體分離測試。此部份目標將針對晶片之表面改質以及晶片液相層析分離 (LC) 與電泳分離 (CE) 進行深入研究。 (III) 質譜接合與質譜分析。此部份整合微流體分離與質譜分析技術並利用獨特的矽晶圓奈米結構吸收質譜儀所發射出的雷射能量，使樣本離子化進行質譜分析。本計畫提案為一獨特的創新構想，目前國內外尚未有研究團隊提出相似的相關研究，且研究未來也將對於了解病理機制、特定生物事件、生物標記、或分子途徑等醫學研究上將有實質的助益。 Proteomics research plays an important role in the study of cells and pathological mechanisms as well as diagnostic cancer biomarkers. However, protein samples are complex and rare. And also, traditional analytical methods are laborious, time-consuming, costly and inefficient. Those reasons make protein analysis a challenge task. Therefore, developing a high efficiency, high sensitivity bio-molecular detection platform to overcome the bottleneck of protein analysis is the main objective of this proposal. In this research, a novel bio-molecular analysis platform named 「Microfluidic separation – mass spectrometry analysis chip (S-MS chip) 」is proposed which integrates sample separation, mass spectrometry and matrix-fee mass sepectrometry interfacing technique into a single microchip format as a simple , high-sensitivity and high-throughput analysis method. Three major research milestones will be completed in this research proposal. (I) Fabrication of S-MS chip. In this research milestone, fabrication process of silicon nanostructure will be developed for chip separation and mass spectrometry analysis. (II) Surface modification and microfluidic separation. In this research milestone, surface treatment, liquid chromatography (LC) separation and capillary electrophoresis (CE) separation will be developed on the S-MS chip platform. (III) Mass spectrometry interfacing and analysis. In this research milestone, chip separation and mass spectrometry techniques will be integrated into a single chip format. By using unique high surface-area ratio S-MS chip surface, biomolecules can be ionized for MS analysis without using matrix solution. 研究期間：9908 ~ 10007
